# ACETYL HEXAPEPTIDE-8 (acetyl-hexapeptide-8) — Global Cosmetic Regulatory & Formulation Monograph

- **Canonical URL**: https://www.inciname.com/en/ingredients/acetyl-hexapeptide-8
- **Standard INCI**: `ACETYL HEXAPEPTIDE-8`
- **KR MFDS Standard Name**: `아세틸헥사펩타이드-8`
- **CAS No.**: `616204-22-9`
- **EC / EINECS No.**: `N/A`
- **Origin**: `peptide`
- **Functions**: 보습제, 피부컨디셔닝제
- **Molecular Formula / MW**: `C34H60N14O12S` (888.99 g/mol)

## 1. 7-Track Official Regulatory Label Names
| Track | Official Label Nomenclature |
| :--- | :--- |
| KR (MFDS Standard) | 아세틸헥사펩타이드-8 |
| KR (Former Names) | — |
| EU (CosIng 1223/2009) | ACETYL HEXAPEPTIDE-8 |
| US (PCPC / MoCRA INCI) | ACETYL HEXAPEPTIDE-8 |
| US (FDA OTC Monograph) | — |
| CN (NMPA IECIC) | 乙酰基六肽-8 |
| JP (JCIA / MHLW QD) | ACETYL HEXAPEPTIDE-8  |

## 2. Layer 1 — Global 6-Region Statutory Summary
- **KR (MFDS)**: `none`
- **EU (1223/2009)**: `none`
- **US (FDA/MoCRA)**: `none`
- **CN (NMPA IECIC)**: `allowed` (Entry: `7780`)
- **JP (MHLW)**: `none`
- **ASEAN (ACD)**: `none`



## 3. Layer 2 — Toxicological & Historical Safe Use Concentrations
- **cn_iecic_max** (leave_on): ≤ 0.5% — NMPA IECIC (2025) Entry 07785 (2025)
- **cn_iecic_max** (rinse_off): ≤ 0.1% — NMPA IECIC (2025) Entry 07785 (2025)

## 4. Layer 3 — Formulation Quartiles & Compatibility
- **Optimal pH Window**: 4 – 7.5
- **Max Process Temp**: ≤ 45°C
- **Synergy Pairings**: PENTAPEPTIDE-18, PALMITOYL TRIPEPTIDE-1, PALMITOYL TETRAPEPTIDE-7, HYDROLYZED SPONGE
- **Incompatibilities**: Hydrophilic hexapeptide (MW 889 Da > 500 Da rule); combine with penetration enhancers (Pentylene Glycol, Propanediol, liposomes, or Spicules) for optimal dermal delivery

## 5. Academic Monograph
■ [1. Chemical Identity & International Labeling Nomenclature]
  - INCI Name: ACETYL HEXAPEPTIDE-8 | CAS No.: 616204-22-9 | EC No.: N/A
  - Origin: PEPTIDE | Functions: , 
  - Physicochemical Profile: Formula C34H60N14O12S | MW 888.99 g/mol | LogP -6.3 | Ionic Nature: amphoteric | PubChem CID 10091330
  - Official Regional Label Names: US [ACETYL HEXAPEPTIDE-8] · EU [ACETYL HEXAPEPTIDE-8] · CN [乙酰基六肽-8] · JP [-] · JP Quasi-Drug [-]
■ [2. Dermatological Mechanism of Action & Functional Role]
  - Biomimetic hexapeptide modeled on the N-terminal domain of SNAP-25. Competitively destabilizes the neuronal SNARE complex required for synaptic vesicle exocytosis, attenuating catecholamine and acetylcholine release to relax dynamic facial expression wrinkles.
■ [3. Global 6-Jurisdiction Regulatory Status & Safety Limits (Layer 1 & Layer 2)]
  - Regional Summary: KR: NONE | EU: NONE | US: NONE | CN: ALLOWED | JP: NONE | ASEAN: NONE (CN IECIC (2025) Entry No. 7780 (乙酰基六肽-8))
  - No specific Annex prohibition or numeric restriction across the 6 major jurisdictions (Permitted under general GMP safety assessment).
  - Official Safety & Historical Use Concentration (Layer 2): CN_IECIC_2025 (cn_iecic_max, leave_on): Max 0.5% / CN_IECIC_2025 (cn_iecic_max, rinse_off): Max 0.1%
■ [4. Formulation Dosage Guide by Product Category (Layer 3-A)]
  - [aqueous_serum] Typical range: 0.005 ~ 0.5% (Median 0.05%, Ref: Lipotec Argireline Peptide Technical Dossier & PubMed) — SNARE complex (SNAP-25) competitive inhibitor for expression wrinkle relaxation
  - [emulsion_cream] Typical range: 0.005 ~ 0.5% (Median 0.05%, Ref: Argireline Clinical Dossier & CN IECIC) — Eye cream & anti-wrinkle neuro-peptide active
■ [5. Formulation Engineering, Stability & Incompatibility Matrix (Layer 3-B)]
  - Addition Phase: Cool-down Phase (< 40°C)
  - Optimal pH Range: pH 4.0 ~ 7.5 | Max Process Temp: Max 45°C | Electrolyte Tolerance: MEDIUM
  - Incompatibility / Caution: Hydrophilic hexapeptide (MW 889 Da > 500 Da rule); combine with penetration enhancers (Pentylene Glycol, Propanediol, liposomes, or Spicules) for optimal dermal delivery
  - Synergy Pairings: PENTAPEPTIDE-18, PALMITOYL TRIPEPTIDE-1, PALMITOYL TETRAPEPTIDE-7, HYDROLYZED SPONGE | Reference Trade Names: Argireline® Peptide, Argireline® Amplified
■ [6. Peer-Reviewed Literature & Official Compendia Evidence]
  - PMID 42084412 (2026, J Craniofac Surg): Ex Vivo Evaluation of Skin Permeability Enhancement Using TargetCool in Human-Derived Skin Tissue Models
  - PMID 41668671 (2026, Int J Cosmet Sci): The effect of a serum containing acetyl hexapeptide-8, dipeptide diaminobutyroyl benzylamide diacetate and gluconolacton
  - PMID 41628877 (2026, Int J Biol Macromol): Thermostable hyaluronic acid-based dissolving microneedles with high-loading capacity: design, optimization, and transde

## 6. Linked PubMed Literature
- [PMID: 42084412] Ex Vivo Evaluation of Skin Permeability Enhancement Using TargetCool in Human-Derived Skin Tissue Models — *J Craniofac Surg* (2026)
- [PMID: 41668671] The effect of a serum containing acetyl hexapeptide-8, dipeptide diaminobutyroyl benzylamide diacetate and gluconolactone on skin biomarkers, wrinkles and skin texture: Ex vivo and clinical studies — *Int J Cosmet Sci* (2026)
- [PMID: 41628877] Thermostable hyaluronic acid-based dissolving microneedles with high-loading capacity: design, optimization, and transdermal delivery of anti-aging ingredients — *Int J Biol Macromol* (2026)
- [PMID: 40715158] Oligomeric hyaluronic acid-modified liposomes effectively improved skin permeability and anti-ageing activity of ellagic acid — *Sci Rep* (2025)
- [PMID: 40673537] Safety Assessment of Acetyl Hexapeptide-8 Amide as Used in Cosmetics — *Int J Toxicol* (2025)
- [PMID: 40626902] Dual-function supramolecular system of α-hydroxy acid-based ionic liquids and peptides for enhanced anti-aging transdermal delivery — *J Mater Chem B* (2025)
- [PMID: 40565185] Acetyl Hexapeptide-8 in Cosmeceuticals-A Review of Skin Permeability and Efficacy — *Int J Mol Sci* (2025)
- [PMID: 40522284] A Supramolecular System of Bioactive Ionic Liquid, Peony Extract, and Peptide for Enhanced Permeability and Synergistic Skincare Benefits — *Chemistry* (2025)
- [PMID: 40196949] Acetyl Hexapeptide-8 as a Topical Alternative to Botulinum Toxin: A Review of the Literature — *J Drugs Dermatol* (2025)
- [PMID: 40176351] BAK and ARG Have Moisturizing, Anti-Inflammatory, Antioxidant, and Antioxidant Bioactivities, in the Zebrafish Model — *J Cosmet Dermatol* (2025)
- [PMID: 39496132] INDIVIDUAL ARTICLE: Real-World Clinical Experience With a Neuro-Peptide Serum in Combination With Botulinum Toxin Type-A Injections — *J Drugs Dermatol* (2024)
- [PMID: 41551769] Fluorous oligoarginines as supra-enhancers for intracellular and transdermal peptide delivery — *Bioact Mater* (2026)